Chachezhania sediminis sp. nov., isolated from marine sediment

A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain (CAU 1508 1 ) was isolated from marine sediment collected in the Republic of Korea. Growth was observed at 10-45 degrees C (optimum, 30 degrees C), pH 4.0-11.0 (optimum, pH 6.0-8.0) and with 0-8.0% (w/v) NaCl (optimum, 2-4%). The isolate formed a monophyletic clade in the phylogenetic analyses using 16S rRNA gene and whole-genome sequences, exhibiting the highest similarity to Chachezhania antarctica SM1703(T) (96.5%), and representing a distinct branch within the genus Chachezhania (family Rhodobacteraceae). Its whole genome sequence was 5.59 Mb long, with a G+C content of 65.7 mol% and 2183 predicted genes belonging to six functional categories. The average nucleotide identity and digital DNA-DNA hybridization values between CAU 1508(T) rand C. antarctica SM1703(T) were 79.1 and 22.2%. respectively. The predominant cellular fatty acids were C-19(:0) cyclo omega 8c and summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two unidentified phospholipids and one unidentified aminophospholipid. The sole isoprenoid quinone was ubiquinone 10. Phenotypic phylogenetic properties supported the classification of CAU 1508(T) as representing a novel species of the genus Chachezhania, with the proposed name Chachezhania sediminis sp. nov. The type strain is CAU 1508(T) (=KCTC 62999(T) =NBRC 11369(T)).

Automated summary

A novel bacterial strain CAU 1508 1 isolated from marine sediment in South Korea belongs to the genus Chachezhania, showing closest similarity to Chachezhania antarctica. It exhibits a wide range of growth conditions in terms of temperature, pH, and salinity, with unique genetic characteristics and fatty acid composition.