Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms22179127
Keywords
influenza virus; RNA modification; epitranscriptome; host-virus interaction; host factor
Funding
- Japan Society for the Promotion of Science [JP19H04832, JP19K07576]
- Japan Agency for Medical Research and Development [20fk0108108h0002]
- Ministry of Education, Culture, Sports, Science and Technology in Japan [16809810]
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Recent studies have shown the importance of RNA modifications in cellular functions, but information about RNA modifications in viral RNA is limited. Influenza A virus infection affects the expression levels of host factors for RNA modifications, some of which may have a proviral effect. This novel aspect of host-virus interactions could lead to the discovery of previously unrecognized viral pathogenicity mechanisms and aid in the development of new antiviral drugs.
Recent studies about the transcriptome-wide presence of RNA modifications have revealed their importance in many cellular functions. Nevertheless, information about RNA modifications in viral RNA is scarce, especially for negative-strand RNA viruses. Here we provide a catalog of RNA modifications including m1A, ac4C, m7G, inosine, and pseudouridine on RNA derived from an influenza A virus infected into A549 cells, as studied by RNA immunoprecipitation followed by deep-sequencing. Possible regions with RNA modifications were found in the negative-strand segments of viral genomic RNA. In addition, our analyses of previously published data revealed that the expression levels of the host factors for RNA modifications were affected by an infection with influenza A virus, and some of the host factors likely have a proviral effect. RNA modification is a novel aspect of host-virus interactions leading to the discovery of previously unrecognized viral pathogenicity mechanisms and has the potential to aid the development of novel antivirals.
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