Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/ijms22116052
Keywords
Chagas disease vector; Rhodnius prolixus; satellite DNA; satellitome; fluorescent in situ hybridization; satellite DNA expression; genome evolution
Funding
- Comision Sectorial de Investigacion Cientifica [160]
- Programa Operativo FEDER Andalucia 2014-2020
- program Plan de Apoyo a la Investigacion 2019-2020, Accion 1 (Univ. Jaen, Spain) [RNM924]
- program Plan de Apoyo a la Investigacion 2017-2018, Accion 6 (Univ. Jaen, Spain)
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Rhodnius prolixus' satellite DNA accounts for 8% of the total genome, composed of 39 satDNA families, including four shared with Triatoma infestans, as well as telomeric and GATA repeats. The clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating complexity in R. prolixus' satellitome, with the four most abundant satDNA families being composed by different subfamilies. Transcription analysis of satDNA families in different tissues showed that 33 out of 39 families are transcribed in four different patterns of expression.
The triatomine Rhodnius prolixus is the main vector of Chagas disease in countries such as Colombia and Venezuela, and the first kissing bug whose genome has been sequenced and assembled. In the repetitive genome fraction (repeatome) of this species, the transposable elements represented 19% of R. prolixus genome, being mostly DNA transposon (Class II elements). However, scarce information has been published regarding another important repeated DNA fraction, the satellite DNA (satDNA), or satellitome. Here, we offer, for the first time, extended data about satellite DNA families in the R. prolixus genome using bioinformatics pipeline based on low-coverage sequencing data. The satellitome of R. prolixus represents 8% of the total genome and it is composed by 39 satDNA families, including four satDNA families that are shared with Triatoma infestans, as well as telomeric (TTAGG)(n) and (GATA)(n) repeats, also present in the T. infestans genome. Only three of them exceed 1% of the genome. Chromosomal hybridization with these satDNA probes showed dispersed signals over the euchromatin of all chromosomes, both in autosomes and sex chromosomes. Moreover, clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating that R. prolixus satellitome is complex and that the four most abundant satDNA families are composed by different subfamilies. Additionally, transcription of satDNA families was analyzed in different tissues, showing that 33 out of 39 satDNA families are transcribed in four different patterns of expression across samples.
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