4.7 Article

Selective Capture and Identification of Methicillin-Resistant Staphylococcus aureus by Combining Aptamer-Modified Magnetic Nanoparticles and Mass Spectrometry

Journal

Publisher

MDPI
DOI: 10.3390/ijms22126571

Keywords

methicillin-resistant Staphylococcus aureus; aptamer; magnetic nanoparticles; antibiotic resistance; MALDI-MS

Funding

  1. Ministry of Science and Technology of the Republic of China [MOST 108-2113-M-259-008]

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A nucleic acid aptamer specific to MRSA has been immobilized on magnetic nanoparticles for capturing bacteria before mass spectrometry analysis, which enhances MRSA identification accuracy. Under optimized conditions, the aptamer-modified nanoparticles showed high capture efficiency for MRSA and low capture rates for non-target bacteria.
A nucleic acid aptamer that specifically recognizes methicillin-resistant Staphylococcus aureus (MRSA) has been immobilized on magnetic nanoparticles to capture the target bacteria prior to mass spectrometry analysis. After the MRSA species were captured, they were further eluted from the nanoparticles and identified using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The combination of aptamer-based capture/enrichment and MS analysis of microorganisms took advantage of the selectivity of both techniques and should enhance the accuracy of MRSA identification. The capture and elution efficiencies for MRSA were optimized by examining factors such as incubation time, temperature, and elution solvents. The aptamer-modified magnetic nanoparticles showed a capture rate of more than 90% under the optimized condition, whereas the capture rates were less than 11% for non-target bacteria. The as-prepared nanoparticles exhibited only a 5% decrease in the capture rate and a 9% decrease in the elution rate after 10 successive cycles of utilization. Most importantly, the aptamer-modified nanoparticles revealed an excellent selectivity towards MRSA in bacterial mixtures. The capture of MRSA at a concentration of 10(2) CFU/mL remained at a good percentage of 82% even when the other two species were at 10(4) times higher concentration (10(6) CFU/mL). Further, the eluted MRSA bacteria were successfully identified using MALDI mass spectrometry.

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