Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms22179302
Keywords
p-coumaric acid; biosensor; laccase; cobalt phtalocyanine
Funding
- Dunarea de Jos University of Galati
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The study introduced a novel enzymatic biosensor, utilizing a screen-printed electrode modified with carbon nanofibers to determine the content of p-coumaric acid. The biosensor showed stable and sensitive responses, with low LOD and LOQ values, and successfully detected p-coumaric acid in phytoproducts of complex composition. The results obtained were comparable to those obtained by the standard spectrometric method.
The present paper developed a new enzymatic biosensor whose support is a screen-printed electrode based on carbon nanofibers modified with cobalt phthalocyanine and laccase (CNF-CoPc-Lac/SPE) to determine the p-coumaric acid (PCA) content by cyclic voltammetry and square wave voltammetry. Sensor modification was achieved by the casting and cross-linking technique, using glutaraldehyde as a reticulation agent. The biosensor's response showed the PCA redox processes in a very stable and sensitive manner. The calibration curve was developed for the concentration range of p-coumaric acid of 0.1-202.5 mu M, using cyclic voltammetry and chronoamperometry. The biosensor yielded optimal results for the linearity range 0.4-6.4 mu M and stood out by low LOD and LOQ values, i.e., 4.83 x 10(-7) M and 1.61 x 10(-)(6) M, respectively. PCA was successfully determined in three phytoproducts of complex composition. The results obtained by the voltammetric method were compared to the ones obtained by the FTIR method. The amount of p-coumaric acid determined by means of CNF-CoPc-Lac/SPE was close to the one obtained by the standard spectrometric method.
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