Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 17, Pages -Publisher
MDPI
DOI: 10.3390/ijms22179335
Keywords
in situ synthesis; hydrogel; scaffold; polyvinyl alcohol; hyaluronic acid; hydroxyapatite; bone tissue engineering; biological evaluation; cell viability
Funding
- Ministry of Education, Youth and Sports of the Czech Republic [CZ.02.1.01/0.0/0.0/16_019/0000787]
- [SVV-2020-260 540]
Ask authors/readers for more resources
Bone tissue engineering aims to mimic the natural behavior of hard tissues. This study found that scaffolds with in situ synthesized hydroxyapatite (HAp) showed better proliferation of human osteoblast-like cells than those with physically mixed pre-prepared HAp, indicating the advantages of in situ synthesizing HAp directly in the organic matrix to improve scaffold properties.
Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available