Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms22168763
Keywords
abasic sites; AP endonucleases; AP lyases; base excision repair; Arabidopsis
Funding
- Spanish Ministry of Science and Innovation (MICINN)
- European Regional Development Fund (FEDER) [PID2019-109967GB-I00]
- University of Cordoba (Plan Propio de Investigacion)
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The study investigated the influence of sequence context on the activity of plant and human AP endonucleases and AP lyases on AP sites opposite G or C, revealing differential preferences and efficiencies of these enzymes in different situations. The findings suggest that plant AP endonucleases and AP lyases may play complementary roles in neutralizing the mutagenic consequences of C deamination and G oxidation on abasic sites formed at C:G pairs.
Abasic (apurinic/apyrimidinic, AP) sites are ubiquitous DNA lesions arising from spontaneous base loss and excision of damaged bases. They may be processed either by AP endonucleases or AP lyases, but the relative roles of these two classes of enzymes are not well understood. We hypothesized that endonucleases and lyases may be differentially influenced by the sequence surrounding the AP site and/or the identity of the orphan base. To test this idea, we analysed the activity of plant and human AP endonucleases and AP lyases on DNA substrates containing an abasic site opposite either G or C in different sequence contexts. AP sites opposite G are common intermediates during the repair of deaminated cytosines, whereas AP sites opposite C frequently arise from oxidized guanines. We found that the major Arabidopsis AP endonuclease (ARP) exhibited a higher efficiency on AP sites opposite G. In contrast, the main plant AP lyase (FPG) showed a greater preference for AP sites opposite C. The major human AP endonuclease (APE1) preferred G as the orphan base, but only in some sequence contexts. We propose that plant AP endonucleases and AP lyases play complementary DNA repair functions on abasic sites arising at C:G pairs, neutralizing the potential mutagenic consequences of C deamination and G oxidation, respectively.
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