Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms22168402
Keywords
alginate; alginate lyase; exo-type; monosaccharide; biofuel; brown algae
Funding
- National Key R&D Program of China [2018YFC0311105]
- Natural Science Foundation of Shandong Province [ZR2018MD021, ZR2019ZD18]
- Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0401-2]
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The novel exo-type alginate lyase VsAly7D isolated from brown algae showed the highest activity at 35 degrees C, with considerable activity at 20 and 30 degrees C, while exhibiting optimal activity under alkaline conditions. VsAly7D demonstrated efficient degradation of alginate, polyM, and polyG, making it a promising tool enzyme for bioethanol production from brown algae.
Brown algae is a kind of renewable resource for biofuels production. As the major component of carbohydrate in the cell walls of brown algae, alginate can be degraded into unsaturated monosaccharide by exo-type alginate lyases, then converted into 4-deoxy-L-erythro-5-hexoseulose uronate (DEH) by a non-enzyme reaction, which is an important raw material for the preparation of bioethanol. In our research, a novel exo-type alginate lyase, VsAly7D, belonging to the PL7 family was isolated from marine bacterium Vibrio sp. QY108 and recombinantly expressed in Escherichia coli. The purified VsAly7D demonstrated the highest activity at 35 degrees C, whereas it still maintained 46.5% and 83.1% of its initial activity at 20 degrees C and 30 degrees C, respectively. In addition, VsAly7D exhibited the maximum activity under alkaline conditions (pH 8.0), with the simultaneously remaining stability between pH 8.0 and 10.0. Compared with other reported exo-type enzymes, VsAly7D could efficiently degrade alginate, poly-beta-D-mannuronate (polyM) and poly-alpha-L-guluronate (polyG) with highest specific activities (663.0 U/mg, 913.6 U/mg and 894.4 U/mg, respectively). These results showed that recombinant VsAly7D is a suitable tool enzyme for unsaturated alginate monosaccharide preparation and holds great promise for producing bioethanol from brown algae.
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