Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/ijms22147454
Keywords
ATAC-seq; cnidarian; RNA-seq; stress response; pre-conditioning; thermal
Funding
- Moore Foundation [4598]
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This study investigated the response differences to heat exposure between lab-cultured and field-conditioned Nematostella vectensis using experimental and gene sequencing techniques. The results showed that field-conditioned animals had a more concentrated response to heat stress, while lab animals had a more diffuse reaction, indicating that pre-conditioning has a significant impact on organisms' response to heat exposure.
Organisms' survival is associated with the ability to respond to natural or anthropogenic environmental stressors. Frequently, these responses involve changes in gene regulation and expression, consequently altering physiology, development, or behavior. Here, we present modifications in response to heat exposure that mimics extreme summertime field conditions of lab-cultured and field-conditioned Nematostella vectensis. Using ATAC-seq and RNA-seq data, we found that field-conditioned animals had a more concentrated reaction to short-term thermal stress, expressed as enrichment of the DNA repair mechanism pathway. By contrast, lab animals had a more diffuse reaction that involved a larger number of differentially expressed genes and enriched pathways, including amino acid metabolism. Our results demonstrate that pre-conditioning affects the ability to respond efficiently to heat exposure in terms of both chromatin accessibility and gene expression and reinforces the importance of experimentally addressing ecological questions in the field.
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