Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 19, Pages -Publisher
MDPI
DOI: 10.3390/ijms221910239
Keywords
wheat; powdery mildew; express sequence; variation annotation; linkage region
Funding
- National Agricultural Park Innovation Project from the Science and Technology Department of Sichuan Province, China [2019YFN0032]
- Applied Basic Research Project from the Science and Technology Department of Sichuan Province, China [2020YJ0331]
- National Natural Science Foundation of China [3210150473]
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This study successfully identified and cloned the Pm40 gene resistant to wheat powdery mildew, demonstrating its effectiveness against the Bgt E20 strain. The gene TraesCS7B01G164000 is suggested as a potential candidate gene for Pm40 based on cosegregation linkage analysis and qRT-PCR results.
Wheat powdery mildew, caused by the obligate parasite Blumeria graminis f. sp. tritici, severely reduces wheat yields. Identifying durable and effective genes against wheat powdery mildew and further transferring them into wheat cultivars is important for finally controlling this disease in wheat production. Pm40 has been widely used in wheat breeding programs in Southwest China due to the spectrum and potentially durable resistance to powdery mildew. In the present study, a resistance test demonstrated that Pm40 is still effective against the Bgt race E20. We identified and cloned the TraesCS7B01G164000 with a total length of 4883 bp, including three exons and two introns, and encoded a protein carrying the CC-NBS-NBS-LRR domain in the Pm40-linked region flanked by two EST markers, BF478514 and BF291338, by integrating analysis of gene annotation in wheat reference genome and both sequence and expression difference in available transcriptome data. Two missense mutations were detected at positions 68 and 83 in the CC domain. The results of both cosegregation linkage analysis and qRT-PCR also suggested that TraesCS7B01G164000 was a potential candidate gene of Pm40. This study allowed us to move toward the final successfully clone and apply Pm40 in wheat resistance improvement by gene engineering.
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