4.7 Article

GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton

Journal

Publisher

MDPI
DOI: 10.3390/ijms22179115

Keywords

trichomes birefringence-like protein; Verticillium wilt resistance; cotton

Funding

  1. Natural Science Foundation of Henan Province of China [202300410548, 162300410070]
  2. State Key Laboratory of Cotton Biology Open Fund [CB2021A07]
  3. Central Public-interest Scientific Institution Basal Research Fund [1610162021005]

Ask authors/readers for more resources

This study revealed that the TBL34 gene in cotton is related to Verticillium wilt (VW) resistance, with different alleles significantly affecting resistance. Differences in amino acid sequences of related proteins impact resistance conversion, suggesting that the TBL34 gene may play a role in regulating resistance to VW in cotton.
Verticillium wilt (VW) is a typical fungal disease affecting the yield and quality of cotton. The Trichome Birefringence-Like protein (TBL) is an acetyltransferase involved in the acetylation process of cell wall polysaccharides. Up to now, there are no reports on whether the TBL gene is related to disease resistance in cotton. In this study, we cloned a cotton TBL34 gene located in the confidence interval of a major VW resistance quantitative trait loci and demonstrated its relationship with VW resistance in cotton. Analyzing the sequence variations in resistant and susceptible accessions detected two elite alleles GhTBL34-2 and GhTBL34-3, mainly presented in resistant cotton lines whose disease index was significantly lower than that of susceptible lines carrying the allele GhTBL34-1. Comparing the TBL34 protein sequences showed that two amino acid differences in the TBL (PMR5N) domain changed the susceptible allele GhTBL34-1 into the resistant allele GhTBL34-2 (GhTBL34-3). Expression analysis showed that the TBL34 was obviously up-regulated by infection of Verticillium dahliae and exogenous treatment of ethylene (ET), and salicylic acid (SA) and jasmonate (JA) in cotton. VIGS experiments demonstrated that silencing of TBL34 reduced VW resistance in cotton. We deduced that the TBL34 gene mediating acetylation of cell wall polysaccharides might be involved in the regulation of resistance to VW in cotton.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available