Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species

Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which function as a barrier against water loss and xenobiotic penetration, while also serving as chemical signals. Plasticity of CHC profiles can vary depending upon numerous biological and environmental factors. Here, we investigated potential sources of variation in CHC profiles of Nilaparvata lugens, Laodelphax striatellus and Sogatella furcifera, which are considered to be the most important rice pests in Asia. CHC profiles were quantified by GC/MS, and factors associated with variations were explored by conducting principal component analysis (PCA). Transcriptomes were further compared under different environmental conditions. The results demonstrated that CHC profiles differ among three species and change with different developmental stages, sexes, temperature, humidity and host plants. Genes involved in cuticular lipid biosynthesis pathways are modulated, which might explain why CHC profiles vary among species under different environments. Our study illustrates some biological and ecological variations in modifying CHC profiles, and the underlying molecular regulation mechanisms of the planthoppers in coping with changes of environmental conditions, which is of great importance for identifying potential vulnerabilities relating to pest ecology and developing novel pest management strategies.

Automated summary

The study investigated the variation in CHC profiles of important rice pests in Asia, revealing differences among species and changes with developmental stages, sexes, temperature, humidity, and host plants. Genes involved in cuticular lipid biosynthesis pathways were found to be modulated, which may explain the variations in CHC profiles among species in different environments. The research highlights the biological and ecological variations in modifying CHC profiles and the molecular regulation mechanisms of planthoppers, providing valuable insights for pest ecology and novel pest management strategies.