Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms22158148
Keywords
modified mRNA; induced pluripotent stem cells; mRNA-based reprogramming; transdifferentiation; therapeutic application
Funding
- Chang Gung Medical Foundation
- Chang Gung Memorial Hospital, Taiwan [CMRPG1H0083]
- Ministry of Science and Technology, Taiwan [109-2314-B-182A-056-MY3, MOST 110-2314-B-182A-137-MY3]
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Modified mRNA (modRNA)-based somatic reprogramming is an effective method for generating pluripotent stem cells and differentiating functional therapeutic cells. This approach has great potential in disease modeling, drug screening, cell transplantation therapy, and regenerative medicine.
Modified mRNA (modRNA)-based somatic reprogramming is an effective and safe approach that overcomes the genomic mutation risk caused by viral integrative methods. It has improved the disadvantages of conventional mRNA and has better stability and immunogenicity. The modRNA molecules encoding multiple pluripotent factors have been applied successfully in reprogramming somatic cells such as fibroblasts, mesenchymal stem cells, and amniotic fluid stem cells to generate pluripotent stem cells (iPSCs). Moreover, it also can be directly used in the terminal differentiation of stem cells and fibroblasts into functional therapeutic cells, which exhibit great promise in disease modeling, drug screening, cell transplantation therapy, and regenerative medicine. In this review, we summarized the reprogramming applications of modified mRNA in iPSC generation and therapeutic applications of functionally differentiated cells.
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