Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/ijms22189840
Keywords
renal ischemia; acute kidney injury; anaesthesia; animal models; surgical clamp
Funding
- National Institute for Health and Medical Research (Institut National de la Sante et de la Recherche Medicale, Inserm) through an ATIP-Avenir Program grant (2018)
- Annee Recherche grant for the Assistance Publique-Hopitaux de Paris (APHP)
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A new technique for inducing renal ischemia in conscious mice was developed, showing rapid and reproducible results. Furthermore, the study revealed the renal protective effect of anesthesia in ischemia-reperfusion injury models, linked to the mitigation of the IRI transcriptomic program.
Ischemia is a common cause of acute kidney injury worldwide, frequently occurring in patients undergoing cardiac surgery or admitted to the intensive care unit (ICU). Thus, ischemia-reperfusion injury (IRI) remains one of the main experimental models for the study of kidney diseases. However, the classical technique, based on non-traumatic surgical clamps, suffers from several limitations. It does not allow the induction of multiple episodes of acute kidney injury (AKI) in the same animal, which would be relevant from a human perspective. It also requires a deep and long sedation, raising the question of potential anaesthesia-related biases. We designed a vascular occluding device that can be activated remotely in conscious mice. We first assessed the intensity and the reproducibility of the acute kidney injury induced by this new device. We finally investigated the role played by the anaesthesia in the IRI models at the histological, functional and transcriptomic levels. We showed that this technique allows the rapid induction of renal ischemia in a repeatable and reproducible manner, breaking several classical limitations. In addition, we used its unique specificities to highlight the renal protective effect conferred by the anaesthesia, related to the mitigation of the IRI transcriptomic program.
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