Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/ijms22115641
Keywords
Acinetobacter baumannii; capsular polysaccharide; K locus; K106; K112; 6-deoxy-l-talose
Funding
- Russian Science Foundation [19-14-00273]
- Australian Research Council (ARC) DECRA Fellowship [DE180101563]
- Russian Science Foundation [19-14-00273] Funding Source: Russian Science Foundation
- Australian Research Council [DE180101563] Funding Source: Australian Research Council
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The whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry different capsular polysaccharide biosynthesis gene clusters, KL106 and KL112 respectively. Both clusters are related to previously described ones and share certain genes. The structures of CPS material from both isolates are different, with the K112 unit containing an α-l-rhamnose side chain not found in the K106 structure.
Whole genome sequences of two Acinetobacter baumannii clinical isolates, 48-1789 and MAR24, revealed that they carry the KL106 and KL112 capsular polysaccharide (CPS) biosynthesis gene clusters, respectively, at the chromosomal K locus. The KL106 and KL112 gene clusters are related to the previously described KL11 and KL83 gene clusters, sharing genes for the synthesis of l-rhamnose (l-Rhap) and 6-deoxy-l-talose (l-6dTalp). CPS material isolated from 48-1789 and MAR24 was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy. The structures of K106 and K112 oligosaccharide repeats (K units) l-6dTalp-(1 -> 3)-D-GlcpNAc tetrasaccharide fragment share the responsible genes in the respective gene clusters. The K106 and K83 CPSs also have the same linkage between K units. The KL112 cluster includes an additional glycosyltransferase gene, Gtr183, and the K112 unit includes alpha l-Rhap side chain that is not found in the K106 structure. K112 further differs in the linkage between K units formed by the Wzy polymerase, and a different wzy gene is found in KL112. However, though both KL106 and KL112 share the atr8 acetyltransferase gene with KL83, only K83 is acetylated.
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