Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 14, Pages -Publisher
MDPI
DOI: 10.3390/ijms22147252
Keywords
red flour beetle; octopamine receptor; locomotion; reproduction
Funding
- National Natural Science Foundation of China [32072491, 31772233]
- Chongqing Natural Science Foundation [cstc2019jcyj-zdxmX0034]
- Chongqing Young Talents Support Program
- Fundamental Research Funds for the Central Universities [XDJK2017A011, SWU115017]
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The study cloned a beta-adrenergic-like octopamine receptor cDNA and demonstrated its functional role in regulating behavior and physiology in the red flour beetle. RNAi assay showed that TcOct beta 2R affects beetle locomotion, mating duration, and fertility.
The neurohormone octopamine regulates many crucial physiological processes in insects and exerts its activity via typical G-protein coupled receptors. The roles of octopamine receptors in regulating behavior and physiology in Coleoptera (beetles) need better understanding. We used the red flour beetle, Tribolium castaneum, as a model species to study the contribution of the octopamine receptor to behavior and physiology. We cloned the cDNA of a beta-adrenergic-like octopamine receptor (TcOct beta 2R). This was heterologously expressed in human embryonic kidney (HEK) 293 cells and was demonstrated to be functional using an in vitro cyclic AMP assay. In an RNAi assay, injection of dsRNA demonstrated that TcOct beta 2R modulates beetle locomotion, mating duration, and fertility. These data present some roles of the octopaminergic signaling system in T. castaneum. Our findings will also help to elucidate the potential functions of individual octopamine receptors in other insects.
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