A Novel Regulator Participating in Nitrogen Removal Process of Bacillus subtilis JD-014

Aerobic denitrification is considered as a promising biological method to eliminate the nitrate contaminants in waterbodies. However, the molecular mechanism of this process varies in different functional bacteria. In this study, the nitrogen removal characteristics for a newly isolated aerobic denitrifier Bacillus subtilis JD-014 were investigated, and the potential functional genes involved in the aerobic denitrification process were further screened through transcriptome analysis. JD-014 exhibited efficient denitrification performance when having sodium succinate as the carbon source with the range of nitrate concentration between 50 and 300 mg/L. Following the transcriptome data, most of the up-regulated differentially expressed genes (DEGs) were associated with cell motility, carbohydrate metabolism, and energy metabolism. Moreover, gene nirsir annotated as sulfite reductase was screened out and further identified as a regulator participating in the nitrogen removal process within JD-014. The findings in present study provide meaningful information in terms of a comprehensive understanding of genetic regulation of nitrogen metabolism, especially for Bacillus strains.

Automated summary

The study investigated the nitrogen removal characteristics of a newly isolated aerobic denitrifier Bacillus subtilis JD-014, showing efficient denitrification performance within a certain range of nitrate concentration when using sodium succinate as the carbon source. Transcriptome analysis revealed that up-regulated differentially expressed genes were associated with cell motility, carbohydrate metabolism, and energy metabolism. Additionally, a regulator gene nirsir was identified as participating in the nitrogen removal process within JD-014.