Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 22, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms22168539
Keywords
T; arundinaceum; sugarcane; sorghum; maize chromosome painting probes; oligo-FISH; ribosomal DNA; chromosome identification; karyotype
Funding
- National Natural Science Foundation of China [31771863]
- Guangxi Key Laboratory of sugarcane biology, Scientific Research Foundation of Graduate School of Fujian Agriculture and Forestry University [324-1122yb056]
- Guangdong Provincial Team of Technical System Innovation for Sugarcane Sisal Hemp Industry [2019KJ104-04]
- State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources
- China Agriculture Research System of MOF and MARA [CARS-20-1-5]
Ask authors/readers for more resources
The study utilized maize chromosome painting probes to identify chromosomes in Tripidium arundinaceum and established its karyotype, representing a crucial step for further cytogenetic research in sugarcane breeding.
Sugarcane is of important economic value for producing sugar and bioethanol. Tripidium arundinaceum (old name: Erianthus arundinaceum) is an intergeneric wild species of sugarcane that has desirable resistance traits for improving sugarcane varieties. However, the scarcity of chromosome markers has hindered the cytogenetic study of T. arundinaceum. Here we applied maize chromosome painting probes (MCPs) to identify chromosomes in sorghum and T. arundinaceum using a repeated fluorescence in situ hybridization (FISH) system. Sequential FISH revealed that these MCPs can be used as reliable chromosome markers for T. arundinaceum, even though T. arundinaceum has diverged from maize over 18 MYs (million years). Using these MCPs, we identified T. arundinaceum chromosomes based on their sequence similarity compared to sorghum and labeled them 1 through 10. Then, the karyotype of T. arundinaceum was established by multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA are localized on chromosomes 5 and 6, respectively, in T. arundinaceum. Altogether, these results represent an essential step for further cytogenetic research of T. arundinaceum in sugarcane breeding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available