Journal
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Volume 112, Issue -, Pages 52-54Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2021.09.006
Keywords
COVID-19; SARS-CoV-2; Variants of concern (VOC); Genotyping; Epidemiology
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This study successfully characterized the transmission dynamics of the Alpha, Beta, and Delta variants of SARS-CoV-2 in Qatar between April and June 2021 using PCR genotyping and Sanger sequencing techniques.
Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of untypeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar. (c) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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