Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 149, Issue 8, Pages 1593-1604Publisher
WILEY
DOI: 10.1002/ijc.33713
Keywords
HER2; human pluripotent stem cells; lung cancer; lung organoid
Categories
Funding
- Japan 12 Society for the Promotion of Science [17H04399, 20K17748]
- Grants-in-Aid for Scientific Research [17H04399, 20K17748] Funding Source: KAKEN
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Utilizing human induced pluripotent stem cells (hiPSCs), researchers established iHER2-hiPSCs and differentiated lung progenitors to form human lung organoids (HLOs), demonstrating that overexpression of HER2 leads to increased morphological irregularity and proliferation capacity in lung tissue, resembling precancerous lesions. HiPSC-derived HLOs serve as a model to study the early tumorigenesis of lung adenocarcinoma and provide insights into the molecular basis of tumor initiation and progression.
Lung adenocarcinoma (LUAD) is the most common types among lung cancers generally arising from terminal airway and understanding of multistep carcinogenesis is crucial to develop novel therapeutic strategy for LUAD. Here we used human induced pluripotent stem cells (hiPSCs) to establish iHER2-hiPSCs in which doxycycline induced the expression of the oncoprotein human epidermal growth factor receptor 2 (HER2)/ERBB2. Lung progenitors that differentiated from iHER2-hiPSCs, which expressed NKX2-1/TTF-1 known as a lung lineage maker, were cocultured with human fetal fibroblast and formed human lung organoids (HLOs) comprising alveolar type 2-like cells. HLOs that overexpressed HER2 transformed to tumor-like structures similar to atypical adenomatous hyperplasia, which is known for lung precancerous lesion and upregulated the activities of oncogenic signaling cascades such as RAS/RAF/MAPK and PI3K/AKT/mTOR. The degree of morphological irregularity and proliferation capacity were significantly higher in HLOs from iHER2-hiPSCs. Moreover, the transcriptome profile of the HLOs shifted from a normal lung tissue-like state to one characteristic of clinical LUAD with HER2 amplification. Our results suggest that hiPSC-derived HLOs may serve as a model to recapitulate the early tumorigenesis of LUAD and would provide new insights into the molecular basis of tumor initiation and progression.
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