Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 183, Issue -, Pages 2305-2313Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.06.027
Keywords
Hemin; G-quadruplex; Electrochemical; Sensors
Funding
- National Science Foundation of China [21665026, 21964018]
- Guangxi Medical High-level Leading Talents Training 139 Project [GWKJ2018-22]
- Special Funding for Guangxi Special Experts [GRCT2019-13]
- Natural Science Foundation of Guangxi Province [2018GXNSFAA138211]
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A label-free homogeneous electrochemical sensor was developed for the detection of apolipoprotein A4 using a proximity hybridization triggered rolling circle amplification induced G-quadruplex formation. This method showed sensitive detection with a wide concentration range and low detection limit, and demonstrated promising potential for clinical diagnosis of depression.
In this paper, we developed a label-free homogeneous electrochemical sensor for detection of apolipoprotein A4 based on proximity hybridization triggered rolling circle amplification induced G-quadruplex formation. The presence of apolipoprotein A4 promoted the formation of a proximate complex via the proximity hybridization of the aptamer DNA strands, which unfolded the molecular beacon, the stem part of molecular beacon as a primer to initiate the RCA process. Thus, with the electrochemical indicator hemin selectively intercalated into the multiple G-quadruplexes, a significant electrochemical signal drop is observed, which is dependent on the concentration of the target apolipoprotein A4. Thus, using this signal-off mode, label-free homogeneous electrochemical strategy for sensitive apolipoprotein A4 assay with a wide range from 1 pg mL(-1) to 100 ng mL(-1), with a low detection limit of 0.51 pg mL(-1). And it rendered satisfactory analytical performance for the deter-mination of apolipoprotein A4 in serum samples. Furthermore, this method also exhibits additional advantages of simplicity and low cost, since both expensive labeling and sophisticated probe immobilization processes are avoided. The satisfactory results indicated that the proposed sensor had promising potential in the clinical diagnosis of depression.
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