Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 185, Issue -, Pages 949-958Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2021.07.004
Keywords
Sesquiterpene synthase; Tripterygium wilfordii; Linalool; nerolidol; geranyllinalool; Substrate promiscuity; Biosynthesis
Funding
- National Natural Science Foundation of China [82003894, 81773830]
- National Key R&D Program of China [2020YFA0908000, 2060302180603]
- National Program for Special Support of Eminent Professionals
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Acyclic terpenes, commonly found in plants, are controlled by different terpene synthases. The study found that terpene synthases from Tripterygium wilfordii exhibited substrate promiscuity and key residues were identified for the generation of diterpene product. Additionally, an engineered yeast producing high levels of nerolidol was constructed for potential applications in microbial fermentation.
Acyclic terpenes, commonly found in plants, are of high physiological importance and commercial value, and their diversity was controlled by different terpene synthases. During the screen of sesquiterpene synthases from Tripterygium wilfordii, we observed that Ses-TwTPS1-1 and Ses-TwTPS2 promiscuously accepted GPP, FPP, and GGPP to produce corresponding terpene alcohols (linalool/nerolidol/geranyllinalool). The Ses-TwTPS1-2, SesTwTPS3, and Ses-TwTPS4 also showed unusual substrate promiscuity by catalyzing GGPP or GPP in addition to FPP as substrate. Furthermore, key residues for the generation of diterpene product, (E, E)-geranyllinalool, were screened depending on mutagenesis studies. The functional analysis of Ses-TwTPS1-1:V199I and Ses-TwTPS1-2: I199V showed that Val in 199 site assisted the produce of diterpene product geranyllinalool by enzyme mutation studies, which indicated that subtle differences away from the active site could alter the product outcome. Moreover, an engineered sesquiterpene high-yielding yeast that produced 162 mg/L nerolidol in shake flask conditions was constructed to quickly identify the function of sesquiterpene synthases in vivo and develop potential applications in microbial fermentation. Our functional characterization of acyclic sesquiterpene synthases will give some insights into the substrate promiscuity of diverse acyclic terpene synthases and provide key residues for expanding the product portfolio.
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