Codon usage patterns and evolution of HSP60 in birds

Heat shock protein 60 (HSP60) is highly conserved from prokaryotic to eukaryotic organisms, acting as molecular chaperone and other vital biological functions. In spite of increasing knowledge of HSP60, its evolutionary mechanism on functional adaption is still far from completely understood. Moreover, analysis of codon usage bias (CUB) is a powerful tool to understand evolutionary association studies. However, so far, as we know, no scientific work on CUB of HSP60 in birds has been reported. In this study, we provide a comprehensive analysis on the codon usage and molecular evolution of HSP60 across birds. The results indicated that HSP60 had a weak codon usage bias with high ENC values (range from 52.66 to 61), low RSCU, and A/T-ending codons were mostly preferred. Meanwhile, it was considered that mutation, natural selection, and genetic drift combined to shape codon usage patterns with different strength proportions among various birds for HSP60. Then, the LRT tests suggested that different lineages of birds might be under similar selective pressures. Besides, the two positive selection sites (151 and 131) were detected and might undergo radical changes. These findings would contribute to understand function diversity and molecular evolution of HSP60 in birds. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study provides a comprehensive analysis on the codon usage and molecular evolution of HSP60 across birds, revealing a weak codon usage bias with high ENC values and A/T-ending codons being preferred. The evolutionary patterns of HSP60 in birds are shaped by mutation, natural selection, and genetic drift, with different lineages possibly under similar selective pressures and positive selection sites undergoing radical changes. This research contributes to a better understanding of the function diversity and molecular evolution of HSP60 in birds.