Polysaccharides as separation media for the separation of proteins, peptides and stereoisomers of amino acids

Reliable separation of peptides, amino acids and proteins as accurate as possible with the maximum conformation and biological activity is crucial and essential for drug discovery. Polysaccharide, as one of the most abundant natural biopolymers with optical activity on earth, is easy to be functionalized due to lots of hydroxyl groups on glucose units. Over the last few decades, polysaccharide derivatives are gradually employed as effective separation media. The highly-ordered helical structure contributes to complex, diverse molecular recognition ability, allowing polysaccharide derivatives to selectively interact with different analytes. This article reviews the development, application and prospects of polysaccharides as separation media in the separation of proteins, peptides and amino acids in recent years. The chiral molecules mechanism, advantages, limitations, development status and challenges faced by polysaccharides as separation media in molecular recognition are summarized. Meanwhile, the direction of its continued development and future prospects are also discussed.

Automated summary

This article reviews the development, application, and prospects of polysaccharides as separation media in recent years for the separation of proteins, peptides, and amino acids. The highly-ordered helical structure of polysaccharide derivatives contributes to complex, diverse molecular recognition ability, allowing them to selectively interact with different analytes. Challenges, advantages, limitations, and future prospects of polysaccharides as separation media in molecular recognition are summarized.