Isolation, structural characterization and neuroprotective activity of exopolysaccharide from Paecilomyces cicada TJJ1213

Two exopolysaccharide fractions (EPS1 and EPS2) were obtained from Paecilomyces cicadae TJJ 1213, and their structures were elucidated. The EPS1 and EPS2were mainly composed ofmannose and galactosewith molar ratios of 3.2: 1.0 and 2.7: 1.0, respectively. They possessed averagemolecular weights of 1.69 x 10(6) and 8.06 x 10(5) Da, respectively. Structural characterization indicated that the backbone of EPS1was consisted of -> 4)-alpha-D-Manp (1 ->, -> 3,4)-alpha-D-Manp (1 -> and -> 2,6)-alpha-D-Manp (1 ->, -> 6)-alpha-D-Galp (1 ->, -> 6)-beta-D-Galp (1 ->, and side chain was consisted of alpha-D-Manp residue. The backbone of EPS2 was composed of beta 6)-beta-D-Galp-(1 ->, -> 4)-alpha-D-Manp-(1 ->, -> 2,6)-alpha-D-Manp-(1 -> and -> 6)-alpha-D-Galp-(1 ->, and the branching point was also consisted of alpha-D-Manp residue. In addition, EPS1 and EPS2 had potential in protective effects of PC12 cells against hydrogen peroxide induced oxidative stress by inhibiting the production of ROS, reducing LDH leakage and alleviating mitochondrial damage. These results indicated that EPS1 and EPS2 might serve as therapeutic agents for neuronal disorders. (c) 2021 Published by Elsevier B.V.

Automated summary

Two exopolysaccharide fractions (EPS1 and EPS2) were isolated from Paecilomyces cicadae TJJ 1213, with mannose and galactose as main components. Both EPS1 and EPS2 showed potential in protecting PC12 cells from oxidative stress and could potentially serve as therapeutic agents for neuronal disorders.