Extraction of chlorophyll and carotenoids loaded into chitosan as potential targeted therapy and bio imaging agents for breast carcinoma

In the current study, the treatment efficacy of ECHCAH was evaluated in vitro studies using cell viability and flow cytometry in human TNBCs. The results here showed significant gradual reduction in growth of TNBCs (MDA231cell lines) after their exposure to serial concentrations for hydrogel assembly (5 mu g/mL to 25 mu g/mL) for 24 and 48 h, representing (86 +/- 1% to 45 +/- 1.5% p < 0.001) and (79 +/- 1.5% to 35 +/- 2.5% p < 0.001) respectively. The flow cytometry showed significant increase in the present of late apoptotic and necrotic cells (64% +/- 1.2 and 27% +/- 0.3 p < 0.001) after 48 h incubation compared to untreated cells (1.13% +/- 0.3 and 4% +/- 0.2 p < 0.001) respectively. It can be summarized that ECHCA inside targeted hydrogel assemblies can inhibit proliferation of cancer cells. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

The current study evaluated the treatment efficacy of ECHCAH in vitro on human triple-negative breast cancer cells, showing a significant inhibitory effect on cell growth at different concentrations. Flow cytometry results indicated an increase in late apoptotic and necrotic cells under the influence of ECHCA, suggesting its potential to inhibit cancer cell proliferation.