Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 98, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2021.107903
Keywords
HOTAIR; miR-222-3p; ADAM10; Chondrocytes; Osteoarthritis
Categories
Funding
- Scientific and technological projects of Henan Province [182102310183]
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The study showed that HOTAIR was highly expressed in human OA cartilages and IL-1 beta-induced OA model in immortalized chondrocytes. By regulating the miR-222-3p/ADAM10 axis, HOTAIR may contribute to IL-1 beta-induced chondrocytes death, inflammation, extracellular matrix degradation, and oxidative stress in OA.
Background: Long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) contributes to cartilage damages including osteoarthritis (OA). While, its role and mechanism in chondrocytes is incompletely clear. Methods: HOTAIR, microRNA (miR)-222-3p and ADAM metalloproteinase-like domain 10 (ADAM10) expressions were detected by real-time quantitative PCR and western blotting. The interaction between miR-222-3p and HOTAIR or ADAM10 was confirmed by dual-luciferase reporter assay. Cell injury was measured by MTS method, flow cytometry, western blotting, enzyme-linked immunosorbent assay for collagen Type II, type X, sex determining region Y-box 9 (SOX9), matrix metalloproteinase (MMP)-13, interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-alpha, and special assay kits for malondialdehyde (MDA), reactive oxygen species (ROS) and superoxide dismutase (SOD). Results: HOTAIR was highly expressed in human OA cartilages and IL-1 beta-induced OA model in immortalized chondrocytes (C-28/I2). Under IL-1 beta stress, blocking HOTAIR was responsible to high mitochondrial activity and low early apoptosis rate, accompanied with increased B cell lymphoma (Bcl)-2 and LC3B-II/I proteins, boosted IL-10 and SOD productions, suppressed cleaved caspase-3 and p62 proteins, and decreased MDA and ROS levels, as well as elevated secretions of Type II collagen, Type X collagen, SOX9, MMP-13, IL-6, and TNF-alpha. Moreover, miR-222-3p was a target of HOTAIR, and its overexpression and knockdown could suppress and aggravate IL-1 beta induced chondrocytes injury. Furthermore, restoring ADAM10, a target gene of miR-222-3p, counteracted the protective role of miR-222-3p upregulation. Conclusion: HOTAIR might contribute to IL-1 beta-induced chondrocytes death, inflammation, extracellular matrix degradation, and oxidative stress in OA via miR-222-3p/ADAM10 axis.
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