Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 95, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2021.107513
Keywords
Natural product; Chrysanthemum zawadskii Herbich var; latilobum; Dendritic cell; Anti-tumor immunity; Polyfunctional T cell; Adjuvant
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF)
- Nuclear R&D Program of Ministry of Science and ICT [NRF-2019R1C1C1002484]
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The polysaccharide CP isolated from Chrysanthemum zawadskii Herbich var. latilobum leaves demonstrated immunostimulatory effects and potential as a vaccine adjuvant. CP-treated DCs induced T cell activation and enhanced immune responses, including specific T cell generation and anti-tumor activity.
The objective of the current study was to demonstrate the immunostimulatory effects of a polysaccharide isolated from Chrysanthemum zawadskii Herbich var. latilobum leaves (CP) and evaluate its potential as a vaccine adjuvant. Results showed that CP induced maturation of the dendritic cells (DCs). In addition, CP-treated DCs activated naive T cells to polarized CD4+ and CD8+ T cells and substantially induced the production of IFN-gamma and IL-2 in vitro. Furthermore, CP initiated the maturation of DCs via the activation of MAPK and NF-xB signaling pathways. Interestingly, systemic administration of CP-treated DCs pulsed with ovalbumin (OVA) peptides significantly enhanced the immune response in vivo, which included the generation of antigen (OVA)-specific polyfunctional T cells, increased cytotoxic T lymphocyte activity, induction of Th1-mediated humoral immunity, and suppression of tumor growth. Taken together, our study highlighted the immunoregulatory activity of CP as well as its potential as a candidate vaccine adjuvant.
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