Journal
INTERNATIONAL IMMUNOPHARMACOLOGY
Volume 97, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.intimp.2021.107677
Keywords
Natural Killer Cells; 5-ALA; NKG2D; Flow Cytometry; qRT-PCR; Cancer
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Funding
- Ahvaz Jundishapur University of Med-ical Sciences
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The study demonstrated that 5-ALA can increase gene expression of NKG2D and the presence of its receptor on NK cells, thereby activating NK cells' killing activity and preventing the growth and metastasis of cancer cells.
Natural killer (NK) cells are involved in innate and acquired immunity, stimulating and enhancing immune responses via secretion of IFN-gamma and TNF-alpha. NKG2D is among the most important NK's stimulant receptors, the ligands of which are elevated on cancerous and virus-infected cells. We analyzed effect of 5-ALA on gene expression and receptor presentation of NKG2D, which is present on peripheral blood NK cells. Mononuclear cells were isolated from the venous blood samples of healthy individuals. RNA extraction and cDNA synthesis were performed after exposure of samples to 5-ALA, and gene expression was evaluated using Real-Time PCR, and the receptor presence rate on the cell surface was evaluated by flow-cytometry analysis. The results showed the gene expression of NKG2D and the presence of its receptor on NK cells were increased.5-ALA can be used to activate NK cells in their killing activity, preventing the growth and metastasis of cancerous cells.
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