Journal
INFECTION AND IMMUNITY
Volume 89, Issue 10, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00072-21
Keywords
Chlamydia; TREM-1; neutrophils; PMNs; hydrosalpinx; hydrometra; Chlamydia trachomatis; genital disease; mouse
Categories
Funding
- Translational Team Science Award (UNC School of Medicine)
- Translational Team Science Award (NC TRaCS)
- NIAID NIH [R01 AI067678]
- STI T32 grant [AI007001]
- Cancer Center Core Support grant [P30 CA016086]
- North Carolina Biotech Center Institutional Support grant [2017-IDG-1025]
- National Institutes of Health [1UM2AI30836-01]
- NCI [5P30CA016086-42]
- NIH [U54-CA156733, P30 DK 034987]
- NIEHS [5 P30 ES010126-17]
- UCRF
- NCBT [2015-IDG-1007]
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This study found that TREM-1,3 plays a significant role in regulating neutrophil migration and the pathogenesis process in the female mouse genital tract, ensuring appropriate immune responses during Chlamydia muridarum infection.
Genital infections with Chlamydia trachomatis can lead to uterine and oviduct tissue damage in the female reproductive tract. Neutrophils are strongly associated with tissue damage during chlamydial infection, while an adaptive CD4 T cell response is necessary to combat infection. Activation of triggering receptor expressed on myeloid cells-1 (TREM-1) on neutrophils has previously been shown to induce and/or enhance degranulation synergistically with Toll-like receptor (TLR) signaling. Additionally, TREM-1 can promote neutrophil transepithelial migration. In this study, we sought to determine the contribution of TREM-1,3 to immunopathology in the female mouse genital tract during Chlamydia muridarum infection. Relative to control mice, trem1,3(-/- )mice had no difference in chlamydial burden or duration of lower-genital-tract infection. We also observed a similar incidence of hydrosalpinx 45days postinfection in trem1,3(-/- )compared to wild-type (WT) mice. However, compared to WT mice, trem1,3(-/-) mice developed significantly fewer hydrometra in uterine horns. Early in infection, trem1,3(-/-) mice displayed a notable decrease in the number of uterine glands containing polymorphonuclear cells and uterine horn lumens had fewer neutrophils, with increased granulocyte colony-stimulating factor (G-CSF). trem1,3(-/- )mice also had reduced erosion of the luminal epithelium. These data indicate that TREM-1,3 contributes to transepithelial neutrophil migration in the uterus and uterine glands, promoting the occurrence of hydrometra in infected mice.
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