4.7 Article

Co-fermentation of sugarcane bagasse hydrolysate and molasses by Clostridium saccharoperbutylacetonicum: Effect on sugar consumption and butanol production

Journal

INDUSTRIAL CROPS AND PRODUCTS
Volume 167, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.indcrop.2021.113512

Keywords

Hemicellulosic hydrolysate; Sugarcane molasses; ABE fermentation; C; saccharoperbutylacetonicum; Biorefinery; Biobutanol

Funding

  1. Brazilian National Council for Scientific and Technological Development (CNPq) [141465/2015-8, 400803/2013-5, 165075/2017-1, 408149/2018-3]
  2. Sao Paulo Research Foundation (FAPESP) [2015/20630-4]
  3. BE-Basic Foundation

Ask authors/readers for more resources

The co-fermentation of mixed sugars for butanol production is a promising approach, and C. saccharoperbutylacetonicum DSM 14923 shows potential and effective application in this process. The study found that the best-performing fermentation medium had a CHH25/SCM75 ratio, with 97% sugar consumption and no inhibitory effect of undissociated acetic acid at pH 5.5-6.5.
Co-fermentation of mixed sugars to produce butanol is an attractive route in sucrochemical production chains. Herein, high-level mixed sugars from sugarcane bagasse hemicellulosic hydrolysate (HH) and molasses (SCM) were investigated as potential substrates for acetone-butanol-ethanol (ABE) production in batch fermentation by Clostridium saccharoperbutylacetonicum DSM 14923. HH produced after hydrothermal pretreatment was concentrated 5-fold and was called concentrated hemicellulosic hydrolysate (CHH). The fermentation media that were investigated consisted solely of CHH and SCM and three CHH-to-SCM ratios were used to provide 30 g/L initial sugar concentrations and furan derivatives lower than 0.1 g/L. For CHH50/SCM50 and CHH75/SCM25, diluting CHH to concentrations of 15 g/L sugars and 22.5 g/L sugars, respectively, which were supplemented with SCM and nutrients, suffered growth inhibition as a function of the concentration of undissociated acid in the medium. The best-performing medium, CHH25/SCM75 (7.5 g/L and 22.5 g/L sugars from CHH and SCM, respectively and about 0.017 g/L furan derivatives), showed 97 % sugar consumption, and in the pH range of 5.5-6.5, undissociated acetic acid was not an inhibitory molecular form to C. saccharoperbutylacetonicum. After 30 h of fermentation, 7.8 and 9.8 g/L butanol and ABE were produced, respectively, which yielded 0.28 and 0.36 g/g. Based on our findings, C. saccharoperbutylacetonicum exhibits its potential and effective application for renewable butanol production by co-fermenting mixed sugars.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available