4.7 Article

Revealing the topochemical and structural changes of poplar lignin during a two-step hydrothermal pretreatment combined with alkali extraction

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Summary: In this study, a two-step hydrothermal pretreatment combined with alkali extraction method was used to deconstruct poplar cell walls for enzymatic hydrolysis, resulting in the removal of a significant amount of hemicelluloses and lignin. Confocal Raman microscopy revealed that hemicelluloses were unevenly removed from cell walls, mainly originating from the secondary wall. Water-soluble hemicelluloses were found to have a more branched structure compared to alkali-soluble hemicelluloses, with a linear backbone of (1 -> 4)-beta-D-Xylp and 4-O-Me-alpha-d-GlcpA side chains identified in hemicelluloses isolated from poplar. This work provides an efficient pathway to convert poplar into fermentable sugars and hemicelluloses with significant yield.

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