Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 49, Issue -, Pages 225-229Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2015.12.043
Keywords
Fish; Carp; 1L-4/13B; Recombinat protein; B cell; Proliferation; IL-4
Funding
- EU FP7 grant [311993]
- Grants-in-Aid for Scientific Research [26450291, 15H06643] Funding Source: KAKEN
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Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM(+) B cells, because after four days of stimulation the IgM(+) fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rclL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM(+) cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B. (C) 2016 Elsevier Ltd. All rights reserved.
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