Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 56, Issue -, Pages 278-285Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.06.041
Keywords
Pelteobagrus fulvidraco; Poly I:C; Fish immunity; Suppression subtractive hybridization
Funding
- Natural Science Foundation of Jiangsu Province [BK20160444]
- Natural Science Research General Program of Jiangsu Provincial Higher Education Institutions [15KJB240002, 12KJA180009]
- Special Guide Fund Project of Agricultural Science and Technology Innovation of Yancheng city [YKN2014022]
- Open Project of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection [JLCBE13002, JLCBE14006]
- Jiangsu Provincial Key Laboratory for Bioresources of Saline Soils [JKLBS2014013, JKLBS2015004]
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The yellow catfish, Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. However, genomic research and resources on this species are largely unavailable and still in infancy. In the present study, we constructed a cDNA library following poly I:C injection to screen for immune response genes in the spleens of P. fulvidraco using suppression subtractive hybridization (SSH). A total of 420 putative expressed sequence tag (EST) clones were identified at 24 h post-injection, which contain 103 genes consisting of 25 immune response genes, 12 cytoskeleton genes, 7 cell cycle and apoptosis genes, 7 respiration and energy metabolism genes, 7 transport genes, 26 metabolism genes, 10 stress response genes, 9 translational regulation genes, and 71 unknown genes. Real-time quantitative reverse transcription-PCR (qRT-PCR) results revealed that a set of randomly selected immune response genes were identified to be up-regulated after 24 h of poly I:C stimulation compared to controls. Our study provides an annotation of immune genes in detail and insight into fish immunity. (C) 2016 Elsevier Ltd. All rights reserved.
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