Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 59, Issue -, Pages 312-322Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2016.10.040
Keywords
Takifugu obscurus; p53; Gene expression; Vibrio alginolyticus; Apoptosis
Funding
- National Natural Science Foundation of China [31100296, 31402324]
- Hainan Applied Technology Research and Development and Demonstration Projects [ZDXM2015025]
- China Postdoctoral Science Foundation [2012M511829]
- Science and technology achievement transformation funds of Ministry of Agriculture [2013GB2E000365]
- Guangdong Provincial Natural Science Foundation [S2011020003256, S2012040008093]
- Scientific and Technological Planning Project of Guangdong Province [2011B020307010, 2012B020307004]
- Science and technology innovation of Higher School Guangdong Province [cxzd114]
- Project of Guangdong Provincial Oceanic and Fishery Administration [A200901B06]
- Scientific and Technological Planning Project of Guangzhou City [11A82090870, 12C432091991]
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The tumor suppressor protein p53 plays a critical role in cell cycle, apoptosis and DNA repair. In this study, the full-length pufferfish p53 (Pf-p53) was obtained, containing an open reading frame of 1095 bp, a 5'UTR of 157 bp and a 3'UTR of 285 bp with a poly (A) tail. The Pf-p53 encoded a polypeptide of 364 amino acids with a theoretical isoelectric point of 8.03 and predicted molecular weight of 40.6 kDa. Pfp53 was ubiquitously expressed in various tissues with a high-level expression in kidney, liver and gill. Vibrio alginolyticus challenge could induce ROS production and disrupt Ca2+ homeostasis, subsequently leading to the induction of DNA damage and apoptosis, while the Vibrio alginolyticus-induced oxidative stress can also increase the non-specific immunity. The pufferfish challenged with Vibrio alginolyticus showed a sharp increase of Pf-p53 transcript in liver. Subcellular localization analysis revealed that Pfp53 was primarily localized in nucleus. Furthermore, overexpression of Pf-p53 in Hela cells could inhibit cell proliferation and the transcriptional activities of the NF-kappa B promoter. Taken together, our results indicated that Pf-p53 may play an important role in the immune response to Vibrio alginolyticus challenge. (C) 2016 Elsevier Ltd. All rights reserved.
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