4.7 Article

Taxonomic profiling of Symbiodiniaceae and bacterial communities associated with Indo-Pacific corals in the Gulf of Thailand using PacBio sequencing of full-length ITS and 16S rRNA genes

Journal

GENOMICS
Volume 113, Issue 4, Pages 2717-2729

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2021.06.001

Keywords

Coral; Microbiome; Coral-associated bacteria; Symbiodiniaceae; 16S rRNA sequencing; PacBio

Funding

  1. National Science and Technology Development Agency (NSTDA) , Thailand [P-17-52364]
  2. UNESCOL'Oreal For Women in Science Fellowship

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This study investigated the structure and diversity of algal and bacterial communities associated with five Indo-Pacific coral species, revealing that host species was a factor influencing the structure and composition of dinoflagellate communities. Different coral hosts were associated with different microbial taxa, and full-length 16S rRNA sequences demonstrated superior performance in achieving species-resolution taxonomic classification of coral-associated microbiota.
Corals live with complex assemblages of microbes including bacteria, the dinoflagellate Symbiodiniaceae, fungi and viruses in a coral holobiont. These coral-associated microorganisms play an important role in their host fitness and survival. Here, we investigated the structure and diversity of algal and bacterial communities associated with five Indo-Pacific coral species, using full-length 16S rRNA and internal transcribed spacer sequences. While the dinoflagellate communities associated with Porites lutea were dominated with Symbiodiniaceae genus Cladocopium, the other four coral hosts were associated mainly with members of the Durusdinium genus, suggesting that host species was one of the underlying factors influencing the structure and composition of dinoflagellate communities associated with corals in the Gulf of Thailand. Alphaproteobacteria dominated the microbiomes of Pocillopora spp. while Pavona frondifera and P. lutea were associated primarily with Gammaproteobacteria. Finally, we demonstrated a superior performance of full-length 16S rRNA sequences in achieving species-resolution taxonomic classification of coral-associated microbiota.

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