4.2 Article

miR-1 Targeted Downregulation of Bcl-2 Increases Chemosensitivity of Lung Cancer Cells

Journal

GENETIC TESTING AND MOLECULAR BIOMARKERS
Volume 25, Issue 8, Pages 540-545

Publisher

MARY ANN LIEBERT, INC
DOI: 10.1089/gtmb.2021.0009

Keywords

lung cancer; Bcl-2; miR-1; chemosensitivity; prognosis

Funding

  1. National Science Foundation of China [82073607]
  2. Highlevel Talent Research Fund of Xiangnan College [066, 6018801]
  3. Excellent youth project of Hunan Education Department [19B529]

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Bcl-2 is upregulated in lung cancer cells, negatively correlated with patient prognosis, while miR-1 affects the chemosensitivity of lung cancer cells by targeting Bcl-2.
Objective: To investigate the expression of B cell lymphoma-2 (Bcl-2) in lung cancer cells and the effect of the miR-1/Bcl-2 axis on the chemosensitivity of lung cancer. Materials and Methods: Real-time quantitative PCR and western blotting were used to detect the expression of Bcl-2 in human embryonic lung fibroblasts and lung cancer cells. The effects of siRNA directed against Bcl-2, in lung cancer tissue samples was detected by immunohistochemistry; these results were used to develop prognostic models. Bioinformatic analyses, dual luciferase reporter gene technology, and western blotting technology were used to explore the targeted regulation of miR-1 on bcl-2. The effect of miR-1 on the chemosensitivity of lung cancer cells was measured using the MTT assay. Results: Compared with human embryonic lung fibroblasts, Bcl-2 was highly expressed in the lung cancer cells, especially in H460 cells. After silencing Bcl-2 with siRNA, the sensitivity of the cells to cisplatin (CDDP) increased. Immunohistochemical results and prognostic analysis revealed that high Bcl-2 expression in lung cancer tissues was negatively correlated with prognosis of lung cancer patients; A dual luciferase reporter assay combined with western blotting confirmed that miR-1 can bind to the Bcl-23 ' UTR region and regulate its expression. Overexpression of miR-1 in lung cancer cells (H460 and A549) increased the sensitivity of these cells to CDDP. Conclusion: Bcl-2 is upregulated in lung cancer cells, which is negatively correlated with the patient prognosis. miR-1 affects the chemosensitivity of lung cancer cells by targeting Bcl-2. These data should provide a theoretical basis for refining the molecular mechanisms of chemoresistance in lung cancer.

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