Journal
FUTURE MICROBIOLOGY
Volume 16, Issue 13, Pages 977-985Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2020-0261
Keywords
CD8(+) T cells; cytokines; IFN-gamma; Paracoccidioides brasiliensis; paracoccidioidomycosis
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil [165822/2017-1, 308746/2013-9]
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The study found that the frequency of CD8(+) T cell subsets differs between patients with acute and chronic forms of paracoccidioidomycosis, but they may respond similarly to Paracoccidioides brasiliensis infection.
Aim: We aimed to verify the frequency of CD8(+) T cell subsets in patients with acute form and chronic form of paracoccidioidomycosis. Material & Methods: Mononuclear cells from paracoccidioidomycosis patients and healthy donors were isolated and phenotyped by flow cytometry. Dendritic cells were pulsed with Paracoccidioides brasiliensis yeast and co-cultures with lymphocytes. Cytokine production was measured by ELISA. Results: Acute form patients present a higher frequency of Tc1 and Tc10 cells, while chronic form patients have more Tc1 and Tc21 cells, compared with healthy controls. In vitro assays showed that P. brasiliensis induced polarization to the Tc17/Tc22 subsets. Conclusion: Our results suggest that CD8(+) T cells can respond in a similar way to P. brasiliensis infection, regardless of the clinical presentation of the disease.
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