Journal
FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL
Volume 28, Issue 4, Pages 285-296Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/10820132211019143
Keywords
Bacterial spores; fruit juices; thermal processing; kinetics; food safety and quality
Categories
Funding
- Directorate General of Research, Technology, and Higher Education Republic Indonesia through Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) [760/UN19.5.1.3/PT.01.03/2019]
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This study investigated the thermal inactivation of B. subtilis and B. licheniformis spores in pineapple juice at different temperatures and soluble solids, and found that the susceptibility of the bacteria to soluble solid change was affected by the interaction between temperature, SS and strain. Pasteurization processes of >= 95 degrees C for >= 33.8 mins were needed to ensure a recommended 5-log reduction of B. subtilis spores and limit vitamin C degradation of pineapple juice within three weeks of storage at 4 degrees C.
Bacillus subtilis and Bacillus licheniformis spores can survive processing temperatures used in the thermal processes of high-acid foods. Therefore, this study investigated the thermal inactivation of B. subtilis and B. licheniformis spores in pineapple juice at different temperatures (85-100 degrees C) and soluble solids (SS, 11-30 degrees Brix). The quality of juices and microbial loads after the thermal treatments during storage at 4 degrees C for 35 days was then checked. A linear decrease in D-value was observed with increasing temperature of treatment. Furthermore, the D-values determined in pineapple juice were: D-90 degrees C=13.2 +/- 0.5 mins, D-95 degrees C = 6.8 +/- 0.9 mins and D-100 degrees C = 2.1 +/- 1.7 mins for B. subtilis spores, and D-85 degrees C = 16.6 +/- 0.4 mins, D-90 degrees C = 7.6 +/- 0.5 mins and D-95 degrees C = 3.6 +/- 1.5 min, for B. licheniformis. Generally, the susceptibility of the bacteria to soluble solid change was affected by the interaction between temperature, SS and strain. In addition, pasteurization processes of >= 95 degrees C for >= 33.8 mins was needed to ensure a recommended 5-log reduction of B. subtilis spores and limit vitamin C degradation of pineapple juice within three-week of storage at 4 degrees C.
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