4.7 Article

Impact of pH on the interaction between soybean protein isolate and oxidized bacterial cellulose at oil-water interface: Dilatational rheological and emulsifying properties

Journal

FOOD HYDROCOLLOIDS
Volume 115, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2021.106609

Keywords

Bacterial cellulose; Soybean protein isolate; Complexes; Emulsions; Stability

Funding

  1. National Natural Science Foundation of China [32072177]
  2. Fundamental Research Funds for the Central Universities [2662018PY060]

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The addition of TOBC can reduce the surface hydrophobicity of SPI at pH 2-9 by partially hindering SPI unfolding, leading to decreased formation of unordered structure. Around the isoelectric point (pH 4.5), the presence of TOBC improves the interfacial adsorption property of SPI with increased balanced interfacial surface pressure (pi) and diffusion rate constant (K-diff) values, while the viscoelastic modulus shows no significant difference. Emulsions stabilized with SPI/TOBC complexes at pH 4-5 exhibit uniform droplets distributions and smaller sizes (<20 μm), providing practical applications in the food industry.
The utilizations of the complexes or coacervates of protein/polysaccharide generated by electrostatic interactions to stabilize Pickering emulsions have attracted increasing attentions. In this work, the effect of pH on the conformational states, interfacial adsorption characteristics and emulsifying properties of soybean protein isolate/oxidized bacterial cellulose (SPI/TOBC) complexes had been investigated. The spectroscopy analysis, including far-UV circular dichroism and intrinsic tryptophan fluorescence spectroscopy, was applied to evaluate protein conformational transitions. The interfacial adsorption behaviors were characterized by using adsorption kinetics and dilatational viscoelasticity modulus analysis. According to spectroscopy analysis, the addition of TOBC reduced surface hydrophobicity of SPI at pH 2-9, since the added TOBC partially hinder SPI unfolding to decrease the formation of unordered structure. Moreover, the interfacial adsorption property of SPI around isoelectric point (pl, pH 4.5) was improved in the presence of TOBC with increased balanced interfacial surface pressure (pi) and diffusion rate constant (K-diff) values, while the viscoelastic modulus had no significant difference, and the emulsions stabilized with SPI/TOBC complexes exhibited uniform droplets distributions and smaller sizes (<20 mu m) at pH 4-5. This work would provide some guidance for the emulsions stabilized by using protein/polysaccharide complexes and has a practical application in food industry.

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