Journal
FOOD CONTROL
Volume 127, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2021.108109
Keywords
Antibiotics; Eggs; Multiclass methods; LC-HRMS; MS; Validation
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Funding
- Italian Ministry of Health [IZSUM RC0032019]
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A multiclass method has been developed and validated for the determination of antimicrobial substances in eggs, covering sixty-three antibiotics from ten families. The method was successfully validated and applied to analyze 100 commercial egg samples, with only 3% containing residues of authorized substances below the relevant Maximum Residue Limits (MRLs).
A multiclass method for the determination of antimicrobial substances in eggs has been developed and validated, covering sixty-three antibiotics belonging to ten families. After extraction with acidified acetonitrile and EDTA, the analytes were injected into a liquid-chromatography high-resolution mass-spectrometry system operating in positive ionization mode. The procedure was successfully validated as confirmatory method evaluating selectivity, linearity, precision, recovery, decision limit, detection capability, limits of detection and quantitation. The here developed is the first method including all the antibiotics with Maximum Residue Limit (MRL) in eggs, except very polar drugs (aminoglycosides and colistins), which require specific protocols. Finally, a wide survey was carried out analyzing 100 commercial eggs samples from local markets. Only 3% contained residues of authorized substances at concentrations lower than the relevant MRLs.
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