Journal
FOOD CHEMISTRY
Volume 357, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129690
Keywords
Scallop gonad protein isolates; Phenolic compounds; Non-covalent interaction; Structural changes; Affinity binding; In vitro digestion
Funding
- Liaoning Revitalization Talents Program [XLYC1907101]
- Science and Technology Innovation Foundation of Dalian City [2019J11CY005]
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This study investigated the interaction mechanism between EGCG and SGPIs, finding that the interaction was mainly dominated by hydrogen bonds and van der Waals forces, with EGCG-treated SGPIs showing higher antioxidant activity. These results provide a theoretical basis for the application of SGPIs-phenolic complexes as functional food additives.
The present study was aimed to investigate the effects of non-covalent interactions between scallop gonad protein isolates (SGPIs) and different concentrations (20, 120 and 240 mu mol/g, protein basis) of four phenolic compounds, such as (-)-epigallocatechin gallate (EGCG), epicatechin 3-gallate (ECG), epigallocatechin (EGC), and catechin (C), regarding the structural and functional properties of the complex. Total sulfhydryl and surface hydrophobicity in SGPIs decreased by nearly 72% and 65% with 240 mu mol/g EGCG, similar but less appreciable changes were produced by EGC, ECG and C. Fluorescence quenching and thermodynamic parameters suggested that hydrogen bond and van der Waals dominated the interaction process between SGPIs and EGCG, and the interaction was further studied by molecular docking. Moreover, EGCG-treated SGPIs digests exerted higher ABTS(+)center dot scavenging activity than that of ECG and EGC-treated SGPIs. These findings are helpful to reveal the binding mechanism of phenolics and SGPIs, and provide a theoretical basis for the application of SGPIs-phenolic complexes as functional food additives.
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