Journal
FOOD CHEMISTRY
Volume 346, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128883
Keywords
Antibacterial packaging system; Bacteriophage T4; Chemical functionalization; Food application
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1D1A1B03029743]
- KIST [2E30350]
- National Research Foundation of Korea [2017R1D1A1B03029743] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A bacteriophage T4 specific to Escherichia coli has been covalently immobilized on a polycaprolactone (PCL) film, showing strong antibacterial effects against E. coli even after external stimulation. The chemically functionalized PCL film exhibited significantly higher bacterial inhibitory effects compared to physically adsorbed phage T4 when used as a packaging film for raw beef inoculated with E. coli O157:H7, suggesting its promising application potential in food packaging materials.
Bacteriophages (phages) have been extensively utilized as antibacterial agents in the food industry because of their host-specificity. However, their application in polymer films has been limited because of the lack of a strong attachment method for phage to the surface. We developed an antibacterial film by covalently immobilizing Escherichia coli (E. coli)-specific phage T4 on a polycaprolactone (PCL) film. The chemical bond formation was confirmed by XPS analysis, and the covalent attachment of phage T4 effectively inhibited E. coli growth even after external stimulation of the film by sonication. When applied as a packaging film for raw beef inoculated with E. coli O157:H7, the chemically functionalized PCL film showed approximately 30-fold higher bacterial inhibitory effects than the film with physically adsorbed phage T4. These results indicate the promising application potential of chemically functionalized PCL film with phage T4 as an antibacterial food packaging material against the foodborne pathogen E. coli.
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