Journal
FOOD CHEMISTRY
Volume 348, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128858
Keywords
Moringa oleifera defatted leaves; RSM; Protein concentrate; Amino acids; Protein quality
Funding
- FEDER funds
- National Funds (FCT/MEC, Fundacao para a Ciencia e Tecnologia) [PTDC/OCE-ETA/32567/2017MOR2020]
- National Funds (FCT/MEC, Ministerio da Educacao e Ciencia) [PTDC/OCE-ETA/32567/2017MOR2020]
- University of Porto under the Erasmus + Program
- University of Bejaia Algeria
Ask authors/readers for more resources
The study aimed to optimize the production of a protein concentrate from Moringa Oleifera defatted leaves through enzymatic extraction and evaluate its nutritional quality and digestibility. The optimized protein concentrate had higher protein content and essential amino acids, meeting the FAO protein quality expectations. The study highlighted the improvement in protein digestibility for the protein concentrate compared to defatted leaves.
The nutritional value and digestibility of leaf proteins is still a major issue. Therefore, the goal of this work was to optimize the production of a protein concentrate (PC) from Moringa Oleifera defatted leaves (MODL) by enzymatic extraction using Viscozyme L and evaluate its nutritional quality and digestibility. Protein extraction conditions were screened using a factorial design. Enzyme/Substrate ratio and pH had no significant effect, whereas, the significant variables, temperature (degrees C), enzyme concentration and incubation time (h) were optimized by central composite design (CCD). PC contained 55.7% of proteins with a balanced amino acid profile when compared with MODL and higher content of essential amino acids (EAAs) (488.6-402.9 mg/g of protein respectively). Improvement on protein digestibility was observed for MODL compared to PC (64.75-99.86% respectively) and higher protein digestibility corrected amino acid score (PDCAAS) (62.10-91.41% respectively). PC meets FAO protein quality expectations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available