Journal
FOOD CHEMISTRY
Volume 346, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128680
Keywords
Whey protein isolate - lotus seedpod proanthocyanidin conjugate; omega-3 fatty acid; Delivery systems; Physiochemical stability; Antioxidation; Flaxseed oil
Funding
- Earmarked Fund for China Agriculture Research System [CARS-14]
- National Natural Science Foundation of China [31771938]
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The study grafted lotus seedpod proanthocyanidin onto whey protein isolate to create nature-derived antioxidant emulsifiers. Experimental results showed that WPI-LSPC conjugate exhibited better antioxidant capacity and thermal stability compared to WPI.
Emulsified omega-3 polyunsaturated fatty acid have expanding application in different food matrix with improved water solubility while still prone to oxidation. Lotus seedpod proanthocyanidin (LSPC) was grafted to whey protein isolate (WPI) to create nature-derived antioxidant emulsifiers. 1HNMR, SDS-PAGE and multiple spectrometry showed that the structure of protein was changed after grafting. DPPH and FRAP measurements showed that WPI-LSPC conjugate (90.53 +/- 1.48% of DPPH scavenging, 691.85 +/- 4.54 mu g/mL for FRAP assay) possessed a much better antioxidant ability than WPI (17.06 +/- 3.34% of DPPH scavenging, 10.43 +/- 0.26 mu g/mL for FRAP assay). Ultrasonic emulsification and DSC experiments showed that WPI-LSPC conjugate were more effective at forming and stabilizing the flaxseed oil emulsions than pure WPI, with higher thermostability. Likewise, low levels of primary and secondary oxidation products were formed for the conjugate than the pure protein in O/W systems after storage, again suggesting WPI-LSPC could be used as fine antioxidant emulsifiers in oxidizing delivery systems.
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