Spectrofluorimetric analyzes of thiamine and riboflavin in monofloral honey varieties of africanized bees (Apis mellifera)

This research aimed to study the validation of a viable and low-cost spectrofluorimetric method, capable of detecting and quantifying B vitamins in floral varieties of bee honey. The analyzes were performed using chemical and operational methods of pre-defined solutions and standards, performed in triplicate and the results expressed as mean +/- standard deviation. The data were recorded in a Microsoft Office Excel spreadsheet (version 2013) and studied using the statistical program Bioestatic version 3.0. The calibration curves of the vitamins showed the linearity and precision of the method. Subsequently, a descriptive analysis of the data was performed, being considered normal in the Lilliefors normality tests. Finally, the data were subjected to Pearson's correlation test, showing positive and strong correlations, with r (Pearson) ranging from 0.7619 to 1. Honey had positive results regarding the detection and quantification of vitamins B1 and B2 through the spectrofluorimetric method.

Automated summary

This research utilized a viable and low-cost spectrofluorimetric method to detect and quantify B vitamins in floral varieties of bee honey. The method showed linearity and precision, and the data were found to be normally distributed. Pearson's correlation test revealed strong positive correlations between the variables studied. Honey had positive results in detecting and quantifying vitamins B1 and B2 using the spectrofluorimetric method.