Journal
FOOD CHEMISTRY
Volume 346, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128881
Keywords
Malus domestica; Calcium-dependent protein kinases; Reactive oxygen species; Induced resistance; Acibenzolar-S-methyl
Funding
- National Natural Science Foundation of China [31801595]
- First-class Discipline Project of Liaoning Province [LNSPXKBD2020305]
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In this study, ASM treatment increased H2O2 content and reduced antioxidant contents in apples, while EGTA + ASM treatment had the opposite effect. ASM treatment enhanced the activities of multiple enzymes but inhibited catalase activity, while EGTA + ASM treatment suppressed the activities of these enzymes. CDPKs play a crucial role in regulating ROS metabolism and inducing resistance in apples through ASM treatment.
This study was carried out to investigate the effect of acibenzolar-S-methyl (ASM) and ethylenebis (oxyethylenenitrilo) tetraacetic acid (EGTA) treatments on calcium-dependent protein kinases (CDPKs) and reactive oxygen species (ROS) metabolism in apples. Postharvest ASM treatment increased H2O2 content, reduced glutathione and ascorbic acid contents, and NADPH oxidase, peroxidase, ascorbate peroxidase, superoxide dismutase and glutathione reductase activities and retarded catalase activity and MdCAT expression in apples. ASM treatment enhanced MdSOD, MdPOD, MdAPX, MdGR, MdCDPK1, MdCDPK4, MdCDPK5, MdCDPK7, and MdCDPK21 expressions in apples. However, EGTA + ASM treatments suppressed H2O2, glutathione and ascorbic acid contents, NADPH oxidase, peroxidase, superoxide dismutase, ascorbate peroxidase and glutathione reductase activities. EGTA + ASM treatments suppressed the selected genes expressions in ROS metabolism and CDPKs, but up-regulated MdCAT expression in apples. These findings suggest that CDPKs play a vital role in regulating ROS metabolism and involve in inducing resistance in apples by ASM.
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