Journal
FOOD CHEMISTRY
Volume 353, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129487
Keywords
Peroxyl radical; Glycosylation; Myofibrillar proteins; N-epsilon-carboxymethyllysine; N-epsilon-carboxyethyllysine
Funding
- Agriculture Research System of China [CARS41Z]
- Science and Technology Project of Nanjing [202002040]
- Jiangsu Province Policy Guidance Program [BX2020008]
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The study found that peroxyl radicals have an impact on the formation of advanced glycation end products during meat processing, potentially altering protein structure and properties through promoting protein oxidation and glycation. Dose-dependency of peroxyl radicals on AGEs formation was also established with different effects of peroxyl radical induction.
During meat processing, two typical advanced glycation end products (AGEs), N-epsilon-carboxymethyllysine (CML) and N-epsilon-carboxyethyllysine (CEL), are generated by free radical induction. However, the impact of peroxyl radicals on myofibrillar proteins (MPs) glycosylation and CML and CEL formation is scarcely reported. In this study, two peroxyl radicals called ROO. and LOO. derived from AAPH (2,2 '-azobis (2-methylpropionamidine) dihydrochloride) and linoleic acid were exposed prior to the Maillard reaction (glucosamine incubation at 37 degrees C for 24 h). Levels of AGEs (CML/CEL), protein oxidation (sulfhydryl/carbonyl), free amino group, surface hydrophobicity, zeta potential, particle size, intrinsic fluorescence intensity and secondary structure were determined. Together with Pearson's correlation, the assumption that free radicals promote MPs oxidation and glycation, alter the aggregation behavior and structure modification, leading to AGEs promotion has been built. In addition, the effect of dose-dependency of peroxyl radical on AGEs has also been established with different effects of peroxyl radical induction.
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