4.7 Article

A neutral polysaccharide with a triple helix structure from ginger: Characterization and immunomodulatory activity

Journal

FOOD CHEMISTRY
Volume 350, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129261

Keywords

Ginger polysaccharide; Structure characterization; alpha-(1 -> 4)-glucan; Triple helix structure; Immunomodulatory activity

Funding

  1. Major Agricultural Applied Technology Innovation Project of Shandong Province in 2018 [39797]
  2. Major Scientific and Technological Innovation Projects of Key R&D Program of Shandong Province [2019JZZY020607]

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A neutral ginger polysaccharide fraction (NGP) isolated from ginger was found to have a low molecular weight primarily consisting of glucose, with immunomodulatory activity on RAW264.7 cells. NGP could enhance macrophage proliferation, increase immune substance production, and potentially serve as an immune agent.
A neutral ginger polysaccharide fraction (NGP) was isolated from ginger, and its primary structures and immunomodulatory activity were investigated. The results showed that NGP had a low molecular weight of 6305 Da. NGP principally consisted of glucose (93.88%) together with minor levels of galactose (3.27%) and arabinose (1.67%). Besides, results of methylation analysis and 1D/2D NMR spectroscopy demonstrated that NGP was alpha-glucan which had the main chain of 1,4-linked alpha-D-Glcp and alpha-D-Glcp residues branched at C-6 position which was different from the common triple helical beta-glucans. NGP also displayed a remarkable immunological activity on the RAW264.7 cells in vitro. It could significantly enhance the proliferation of macrophages without cytotoxicity and increase the production of immune substances (NO, TNF-alpha, IL-1 beta and IL-6). The secretion at the concentration of 200 mu g/mL was 29.41 mu M, 1496.71, 44.30 and 1889.83 pg/mL for each substance, respectively. The results indicated that NGP could be a potential immune agent and might provide meaningful information for further chain conformation and immune mechanism research.

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