Development of a magnetic MOF-based M-D-μSPE methodology combined with LC-MS/MS for the determination of fluorotelomer alcohols and its metabolites in animal derived foods

In this study, a novel modified metal organic framework (MOF) was prepared and used as adsorbent of miniaturized solid-phase extraction (M-D-mu SPE) for analyzing 8-2 FTOH and its metabolites in edible tissues by LC-MS/MS. This synthesized adsorbent, named as Fe3O4@Fe-MIL 101-NH2 (magnetic Fe-MOF), was characterized. Moreover, the effects factors on the adsorption behavior of the adsorbents for the analytes were investigated and optimized in detail, such as solution pH, adsorbent amount, extraction time, desorption condition. The adsorbtion mechanism of magnetic Fe-MOF might be electrostatic interaction, CF-pi hydrophobic and Lewis acid base. Compared with conventional adsorbents (such as PSA, C18), magnetic Fe-MOF reduced matrix effect. The limits of quantification ranged 0.10-1.5 mu g/kg. The recoveries of analytes ranged 78.0% - 90.3% in spiked samples, with relative standard deviations less than 12.0%. The developed method was successfully utilized to analyze incurred samples, which proves that it is a rapid, efficient, and sensitive method.

Automated summary

A novel modified metal organic framework (MOF) named Fe3O4@Fe-MIL 101-NH2 (magnetic Fe-MOF) was prepared and used as an adsorbent in miniaturized solid-phase extraction (M-D-mu SPE) for the analysis of 8-2 FTOH and its metabolites in edible tissues by LC-MS/MS. The synthesized adsorbent showed good adsorption performance and reduced matrix effect compared to conventional adsorbents, with quantification limits ranging from 0.10-1.5 μg/kg and recoveries of analytes ranging from 78.0%-90.3% in spiked samples, with relative standard deviations less than 12.0%. This developed method proved to be rapid, efficient, and sensitive for analyzing incurred samples.